Reverse vaccinology is a cutting-edge method using genomic information, proteomics, and bioinformatics to select vaccine targets even before studying pathogens in detail. By sequencing the genome of the pathogen, its analysis, and immunological validation, it is possible to rapidly and cheaply identify the candidates for vaccine production. This has been effectively used in diseases such as meningitis and malaria. With tools such as NERVE and Vaxign, reverse vaccinology allows for faster target identification and can be used with pathogens that are hard to culture. The World Vaccines R&D Conference is pivotal in developing this innovative research and creating a global collaboration for efficient vaccine development.